Measurement of bacterial production in stream-bed sediments via leucine incorporation
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چکیده
منابع مشابه
Assessment of [h]thymidine incorporation into DNA as a method to determine bacterial productivity in stream bed sediments.
We performed several checks on the underlying assumptions and procedures of the thymidine technique applied to stream bed sediments. Bacterial production rates were not altered when sediments were mixed to form a slurry. Incubation temperature did affect production rates. Controls fixed and washed with formaldehyde had lower backgrounds than trichloroacetic acid controls. DNA extraction by base...
متن کاملEstimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine.
We examined the simultaneous incorporation of [H]thymidine and [C]leucine to obtain two independent indices of bacterial production (DNA and protein syntheses) in a single incubation. Incorporation rates of leucine estimated by the dual-label method were generally higher than those obtained by the single-label method, but the differences were small (dual/single = 1.1 +/- 0.2 [mean +/- standard ...
متن کامل12 Measuring Bacterial Biomass Production and Growth Rates from Leucine Incorporation in Natural Aquatic Environments
The rate of biomass production is a fundamental property of all organisms in nature, but it is an especially important parameter of microbes in natural aquatic environments. An estimate of microbial production can be used as a general index of microbial activity and specifically to calculate growth rates. Since many processes scale with it, biomass production can be used to obtain a first-order...
متن کاملBacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates
The thymidine and leucine methods were examined and used to estimate bacterial production in the Delaware Estuary. During growth experiments that minimized grazing on bacteria, conversion factors for both thymidine and leucine were initially high and then rapidly decreased to values lower than commonly-used factors (2.0 X 10" cells mol-' for thymidine). The low thymidine conversion factors may ...
متن کاملContribution of major bacterial groups to bacterial biomass production (thymidine and leucine incorporation) in the Delaware estuary
Assimilation of 3H-thymidine and 3H-leucine was examined at the single-cell level using a combination of microautoradiography and fluorescent in situ hybridization (Micro-FISH) to determine the contribution of various bacterial groups to bacterial production in aquatic systems. All of the major phylogenetic groups of bacteria examined along the salinity gradient of the Delaware estuary, includi...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 1996
ISSN: 0168-6496
DOI: 10.1016/s0168-6496(96)00067-0